陈家良 博士 高聘研究员 博（硕）士生导师

  邮箱：jialiangchen@hainanu.edu.cn

 办公地址：海南大学美安药学大楼345-5

 1.教育及工作背景

 2024-至今 海南大学 药学院 高聘研究员

 2022-2024 枣庄学院 化学化工与材料科学学院 副教授

 2019-2022 南开大学 化学学院 博士后

 2017-2019 南开大学 生命科学院 博士后

 2010-2016 南开大学 化学院 有机化学博士

 2006-2010 山东师范大学 化学化工与材料科学学院 理学学士

2.科研情况

围绕蛋白质溶液动态结构与功能关系研究中的技术难点，发展顺磁核磁共振新方法，并用于阐释多结构域蛋白质在溶液中的动态结构变化及其与功能的关系、蛋白酶在催化反应过程中短寿命中间体的三维结构及催化反应分子机制；利用顺磁核磁共振技术分析药物分子和天然产物的立体化学结构。近年来发表SCI论文20余篇，其中以第一作者在Angew. Chem. Int. Ed., ACS Catal., Sci. China Chem., J. Phy. Chem. Lett., Chem. Commun.等期刊发表多篇论文。主持国家自然科学基金面上、青年基金项目、山东省青创团队项目，2017年入选全国博士后“博新计划”项目。

3.研究领域

蛋白质动态结构-功能关系、基于蛋白质动态结构的药物分子筛选、代谢物定量分析检测、手性药物分子立体构型分析

4. 社会兼职

Analytical Chemistry, Biosensors & Bioelectronics等学术期刊审稿人

5.代表性项目

1）国家自然科学基金面上项目（22374126）：利用赝接触位移确定手性有机分子的立体构型，2024-2027，46万，主持；

2）国家自然科学基金青年项目（22004072）：细胞内定点标记蛋白质并应用于高分辨生物磁共振研究，2021-2023，24万，主持；

3）山东省高校“青创团队计划”项目（2022KJ279）：重大疾病代谢物的多模态原位定量分析，2023-2025，30万，主持；

4）2017年全国博士后创新人才支持计划-“博新计划”（BX201700124），60万，主持。

6.代表性成果

1) Jia-Liang Chen, Bin Li, Feng Yang, Tiesheng Shi, Xun-Cheng Su. Increase of chiral sensing ability in host-guest chemistry by magnetic anisotropy. Sci. China Chem., 2024, 67, 2775-2782.

2) Jia-Liang Chen, Xiao Wang, Feng Yang, Bin Li, Gottfried Otting, Xun-Cheng Su. 3D structure of the transient intermediate of the enzyme−substrate complex of sortase A reveals how calcium binding and substrate recognition cooperate in substrate activation. ACS Catal., 2023, 13, 11610-11624.

3) Jia-Liang Chen; Yin Yang, Tiesheng Shi, Xun-Cheng Su. Effective assessment of lanthanide ion delivery into live cells by paramagnetic NMR spectroscopy. Chem. Commun., 2023, 59, 10552-10555.

4) Ya-Ting Chen, Bin Li, Jia-Liang Chen, Xun-Cheng Su. Stereospecific recognition of a chiral centre over multiple flexible covalent bonds by ¹⁹F-NMR. Analyst, 2023, 148, 233-238.

5) Jia-Liang Chen, Bin Li, Bo Ma, Xun-Cheng Su. Distinct stereospecific effect of chiral tether between a tag and protein on the rigidity of paramagnetic tag. J. Biomol. NMR, 2022, 76, 107-119.

6) Bo Ma†, Jia-Liang Chen†, Chao-Yu Cui†, Feng Yang, Yan-Jun Gong, and Xun-Cheng Su. Rigid, highly reactive and stable DOTA-like tags containing a thiol-specific phenylsulfonyl pyridine moiety for protein modification and NMR analysis. Chem. Eur. J. 2021, 27, 16145-16152.

7) Jia-Liang Chen, Ben-Guang Chen, Bin Li, Feng Yang and Xun-Cheng Su. Assessing multiple conformations of lanthanide inding tags for proteins using a sensitive ¹⁹F-reporter. Chem. Commun. 2021, 57, 4291-4294.

8) Ya-Ting Chen†, Bin Li†, Xia-Yan Li, Jia-Liang Chen, Chao-Yu Cui, Kaifeng Hu, Xun-Cheng Su. Simultaneous identification and quantification of amino acids in biofluids by reactive ¹⁹F-tags. Chem. Commun. 2021, 57, 13154-13157.

9) Wence Xu†, Guilan Fan†, Jialiang Chen, Jinhan Li, Le Zhang, Shengli Zhu, Xuncheng Su, Fangyi Cheng and Jun Chen. Nanoporous palladium hydride for electrocatalytic N₂ reduction under ambient conditions. Angew. Chem. Int. Ed. 2020, 59, 3511-3516.

10) Qiang Li, Jialiang Chen, Marko Trajkovski, Yifei Zhou, Chaochao Fan, Kuan Lu, Pingping Tang, Xuncheng Su, Janez Plavec, Zhen Xi and Chuanzheng Zhou. 4′-Fluorinated RNA: synthesis, structure, and applications as an ultra-sensitive ¹⁹F NMR probe of RNA structure and function. J. Am. Chem. Soc. 2020, 142, 4739-4748.

11) Jia-Liang Chen†, Bin Li†, Xia-Yan Li, and Xun-Cheng Su. Dynamic exchange of the metal chelating moiety: a key factor in determining the rigidity of protein−tag conjugates in paramagnetic NMR. J. Phys. Chem. Lett. 2020, 11, 9493-9500.

12) Xun-Cheng Su*, Jia-Liang Chen. Site-specific tagging of proteins with paramagnetic ions for determination of protein structures in solution and in cells. Acc. Chem. Res. 2019, 52, 1675-1686.

13) Jia-Liang Chen, Xiao Wang, Yu-Hao Xiao and Xun-Cheng Su. Resonance assignments of lowly populated and unstable enzyme intermediate complex under real-time conditions. ChemBioChem, 2019, 20, 2738-2742.

14) Mingming Li†, Yashan Ning†, Jialiang Chen, Xingchen Duan, Na Song, Dan Ding, Xuncheng Su, and Zhilin Yu. Proline isomerization-regulated tumor microenvironment adaptable self-assembly of peptides for enhanced therapeutic efficacy. Nano Lett. 2019, 19, 7965-7976.

15) Xiao Wang, Jia-Liang Chen, Gottfried Otting, Xun-Cheng Su. Conversion of an amide to a high-energy thioester by Staphylococcus aureus sortase A is powered by variable binding affinity for calcium. Sci. Rep. 2018, 8, 16371.

16) Jia‑Liang Chen†, Yu Zhao†, Yan‑Jun Gong, Bin‑Bin Pan, Xiao Wang, Xun‑Cheng Su. Stable and rigid DTPA-like paramagnetic tags suitable for in vitro and in situ protein NMR analysis. J. Biomol. NMR, 2018, 70, 77-92.

17) Yin Yang†, Feng Yang†, Yan-Jun Gong, Jia-Liang Chen, Daniella Goldfarb, and XunCheng Su. A reactive, rigid Gd^III labeling tag for in-cell EPR distance measurements in proteins. Angew. Chem. Int. Ed. 2017, 56, 2914-2918.

18) Jia-Liang Chen, Xiao Wang, Feng Yang, Chan Cao, Gottfried Otting, and Xun-Cheng Su. 3D structure determination of an unstable transient enzyme intermediate by paramagnetic NMR spectroscopy. Angew. Chem. Int. Ed. 2016, 55, 13744-13748.

19) Jia-Liang Chen, Yin Yang, Lin-Lin Zhang, Haobo Liang, Thomas Huber, Xun-Cheng Su and Gottfried Otting. Analysis of the solution conformations of T4 lysozyme by paramagnetic NMR spectroscopy. Phys. Chem. Chem. Phys. 2016, 18, 5850-5859.

20) Fei-He Ma†, Jia-Liang Chen†, Qing-Feng Li†, Hui-Hui Zuo, Feng Huang, and Xun-Cheng Su. Kinetic assay of the michael addition-like thiol–ene reaction and insight into protein bioconjugation. Chem. Asian J. 2014, 9, 1808-1816.

欢迎分子生物学、核磁共振、化学生物学、合成化学背景的研究生和博士加盟！

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